GREAT AT SMALL THINGS

0

LMG media details

Last data update: 24 January 2024 16:39 CET  

1 medium found

Name:

Medium number: 42

Recipe: (NH4)2SO4 1,3 g

MgCl2 x 6H2O 2,6 g

KH2PO4 1,43 g

K2HPO4 5,5 g

CaCl2 x 2H2O 0,13 g

Sodium-alpha-glycerophophate 6 g

FeSO4 x 7H2O 1,1 mg

Glutathione 0,25 g

Yeast extract 4,5 g

Resazurin 1 mg

Cellulose (Avicel) 10 g

Agar 15 g

Distilled water 1 L

pH 7,1

Prepare under 95% N2 + 5% CO2 gas atmosphere and autoclave. Alternatively, cellobiose can be used instead of cellulose. If so, aseptically add 50 ml of a filter-sterilized 10 % cellobiose solution per liter autoclaved medium.