Last data update: 24 January 2024 16:39 CET
Plasmid name: Caspase-3 6xHis pET23b (LMBP 9099)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
Human caspase 3 cDNA (CASP3, CPP32, GeneID 836) Histidine tag (His-tag); C-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | pET23b |
| EMBL Accession number: | - |
| Latest sequence update: | 16/05/2023 |
| Authenticity test: | The plasmid has been fully sequenced but the NGS data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Salvesen(1). (1) Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA |
| Plasmid reference: | - |
| Related plasmid reference: | Denault and Salvesen, in 'Current protocols in protein science - Expression, purification, and characterization of caspases, 21', Wiley, New York (2003), chapter 21.13 [ISSN/ISBN: 1934-3655] [PMID: 18429241] [DOI: 10.1002/0471140864.ps2113s30] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
Caspase-3 6xHis pET23b (LMBP 9099) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Salvesen. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.