Last data update: 24 January 2024 16:39 CET
Plasmid name: Phasmid ISceIM (LMBP 11286)
New search | Print data sheet |
Price category: | Cat. 3 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p11286.gb
(View with Genome Compiler) p11286.txt p11286.pdf |
Sequence analysis results Genecorner: |
NGS: d03-gc-dec2019.fasta |
Cloned DNA: |
Synthetic I-SceI meganuclease cDNA (I-SceI Meganuclease) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Phage TM4 genome; cDNA |
Promoter: | Mycobacterial optimized promoter (MOP) Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T1 terminator |
Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli (as a plasmid) Mycobacterium (as a phage) |
Parental clone: | suicide vector ISceIM; phAE159 |
Further information: | This phasmid was constructed by isolating the transposon cassette from suicide vector ISceIM as a PacI fragment and ligating it into the PacI opened phAE159 phasmid. Use a lambda packaging protocol to transform E. coli. phAE159 is a temperature-sensitive TM4-based Mycobacterial shuttle plasmid. The nucleotide sequence of the phAE159 phasmid corresponds with the EMBL Nucleotide Sequence Database accession number AF068845.1, except for a number of nucleotide substitutions shown by NGS. Other name of the plasmid is phAE159::suicide vector ISceIM (MOP-ISceIM). |
EMBL Accession number: | AF068845.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 31/08/2020 |
Authenticity test: | This plasmid has been fully sequenced. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Borgers(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
Plasmid reference: | Borgers et al., mSystems 5 (2020), e00180-20 [PMID: 32788404] [DOI: 10.1128/mSystems.00180-20] |
Restricted distribution: | - BCCM MTA |
Distributed as: | active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + hygromycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
Phasmid ISceIM (LMBP 11286) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Borgers et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.