Last data update: 24 January 2024 16:39 CET
Plasmid name: R100drd1 (LMBP 8004)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam) Streptomycin (Sm; spectinomycin (Sp)) Tetracycline (tet) Fuschin acid (fa) Mercuric ion (Hg) Sulfonamide (Sul) |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | The plasmid was constructed in vivo based on natural plasmids. The plasmid contains a derepressed mutant for transfer and pilus synthesis. The plasmid belongs to the IncFII incompatibility family. Approximate size of the plasmid is 105 kb. The plasmid carries the sulI gene, resulting in sulphonamide resistance. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Lawn et al., Nature 216 (1967), 343-346 [PMID: 4861358] [DOI: 10.1038/216343a0] Radström et al., Antimicrob. Agents Chemother. 35 (1991), 1840-1848 [PMID: 1952855] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 GC905 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) + streptomycin (25 μg/ml) + tetracycline (10 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with streptomycin and tetracycline. |
| Other culture collection numbers: | SCK CM0228 |
Refer in your Materials and Methods: |
R100drd1 (LMBP 8004) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.