Last data update: 24 January 2024 16:39 CET
Plasmid name: R135 (LMBP 7975)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Gentamicin (Gm) Streptomycin (Sm) Tetracycline (tet) Sulfonamide (Sul) Methyl-β-D-galactopyranoside (Tra+) |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | R135 is a natural plasmid. It is a single covalently closed self-transmissible plasmid of approximately 60 Mdal in size. This is a reference strain for the IncM incompatibility family. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Natural plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | Witchitz, J. Antibiotics 25 (1972), 622 [PMID: 4567455] |
| Related plasmid reference: | Van Nhieu et al., Antimicrob. Agents Chemother. 29 (1986), 833-837 [PMID: 3015007] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture |
| Host for distribution: | Escherichia coli K12 J53 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + gentamicin (10 μg/ml) + streptomycin (25 μg/ml) + tetracycline (10 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on gentamicin; subsequent cultivation of a single colony in liquid medium with streptomycin and tetracycline. |
| Other culture collection numbers: | SCK CM0116 |
Refer in your Materials and Methods: |
R135 (LMBP 7975) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost and was published in Witchitz, 1972. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.