Last data update: 24 January 2024 16:39 CET
Plasmid name: p110huIL6P-luc+ (LMBP 4693)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4693.gb
(View with Genome Compiler) p4693.txt p4693.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+)) |
| Promoter: | Human interleukin 6 promoter (IL6); 110 bp fragment |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pMahIL6P |
| Further information: | This plasmid contains a 110 bp Sau96I/XhoI fragment of the human IL6 promoter upstream of the mutated firefly luciferase gene. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727162.1. The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. |
| EMBL Accession number: | AC073072.11, view at EMBL, GenBank, DDBJ LT727162.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/10/2011 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, HindII, NcoI, SalI and ScaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Vanden Berghe et al., J. Biol. Chem. 273 (1998), 3285-3290 [PMID: 9452444] |
| Related plasmid reference: | Sherf et al., Promega Notes Magazine 49 (1994), 14-21 Vanden Berghe et al., J. Biol. Chem. 274 (1999), 32091-32098 [PMID: 10542243] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p110huIL6P-luc+ (LMBP 4693) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman and was published in Vanden Berghe et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.