Last data update: 24 January 2024 16:39 CET
Plasmid name: pBABE-Flag-mIL1RAcP (LMBP 13063)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interleukin 1 receptor accessory protein cDNA (Il1rap, IL-1RAcP, GeneID 16180) FLAG epitope tag; N-terminal Moloney murine leukemia virus Pr65 cDNA (gag, GeneID 1491870); truncated |
| Promoter: | Moloney mouse leukemia virus long terminal repeat (5' MMLV-LTR) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR) |
| Selection marker: | Ampicillin (amp) Puromycin (puro) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Copy number: | High copy number |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pBABE-puro |
| Further information: | Retroviral expression vector |
| EMBL Accession number: | - |
| Latest sequence update: | 12/09/2022 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr H. Braun(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Morgenstern and Land, Nucleic Acids Res. 18 (1990), 3587-3596 [PMID: 2194165] [DOI: 10.1093/nar/18.12.3587] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBABE-Flag-mIL1RAcP (LMBP 13063) is available at BCCM/GeneCorner. This plasmid was deposited by Dr H. Braun and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.