Last data update: 24 January 2024 16:39 CET
Plasmid name: pBAD24-ClsA-His10 (LMBP 12406)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli cardiolipin synthase A cDNA (clsA, GeneID 945821) Escherichia coli DNA-binding transcriptional dual regulator AraC cDNA (araC, GeneID 944780) Histidine tag (His-tag); C-terminal |
| Promoter: | Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBAD24 |
| Further information: | The plasmid was constructed by cloning the E. coli ClsA coding sequence with C-terminal His-tag into the pBAD24 vector. The His-tag in this plasmid consists of 10 histidine residues. The nucleotide sequence of the parental pBAD24 vector corresponds with the EMBL Nucleotide Sequence Database accession number X81837.1. Other name of the plasmid is pDC350. |
| EMBL Accession number: | X81837.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/03/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1). (1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Plasmid reference: | Romantsov et al., Mol. Microbiol. 107 (2018), 623-638 [PMID: 29280215] [DOI: 10.1111/mmi.13904] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 WG350 (LMBP 12428) |
| Host reference: | Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBAD24-ClsA-His10 (LMBP 12406) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Romantsov et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.