Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-mABIN-1mER/AA (LMBP 5887)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1); mutated sequence E-tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGS-E-mABIN-1 |
| Further information: | The mouse ABIN-1 gene contains the DFxxER motif. ER/AA mutations in this motif were introduced by site-directed mutagenesis. Other name of the plasmid is pCAGGS-E-mABIN-1 ER/AA. |
| EMBL Accession number: | BC008665, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 11/02/2009 |
| Sequence detail: | Primers used to introduce the ER/AA mutations:
483 485 489 490 495 496
forward: 5' GAA.GAG.GAC.TTC.CAG.AGG.GCA.GCG.AGT.GAC.CGT.GAA.CGC.ATG 3'
Glu Glu Asp Phe Gln Arg Ala Ala Ser Asp Arg Glu Arg Met
^ ^^
reverse: 5' CAT.GCG.TTC.ACG.GTC.ACT.CGC.TGC.CCT.CTG.GAA.GTC.CTC.TTC 3'
^^ ^
wt sequence of mABIN-1:
483 485 489 490 495 496
5' ... GAA.GAG.GAC.TTC.CAG.AGG.GAA.CGG.AGT.GAC.CGT.GAA.CGC.ATG ... 3'
Glu Glu Asp Phe Gln Arg Glu Arg Ser Asp Arg Glu Arg Met
^: mutated nucleotide.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr S. Wagner(1) and received from Dr I. Dikic(1). (1) Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt, Germany |
| Plasmid reference: | - |
| Related plasmid reference: | Wagner et al., Oncogene 27 (2008), 3739-3745 [PMID: 18212736] [DOI: 10.1038/sj.onc.1211042] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-mABIN-1mER/AA (LMBP 5887) is available at BCCM/GeneCorner. The plasmid was deposited by Dr S. Wagner and received from Dr I. Dikic. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.