Last data update: 24 January 2024 16:39 CET
Plasmid name: pCLF4 (LMBP 9392)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9392.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid R6K origin; defective pir gene |
| Host range: | Escherichia coli; use strains supplying the R6K pir function |
| Parental clone: | pKD4 |
| Further information: | The plasmid was constructed by inserting the T7 promoter sequence into the pKD4 vector (B. L. Wanner lab). The plasmid is a template for an FRT-flanked kanamycin resistance cassette. After FLP-mediated excision of the kanamycin resistance gene, the plasmid resulting from pCLF4 is identical to those resulting from pCLF1, pCLF2 and pCLF3. The remaining FLP recognition site has termination codons in all 6 reading frames. The nucleotide sequence of the pCLF4 vector corresponds with the EMBL Nucleotide Sequence Database accession number EU629214.1. |
| EMBL Accession number: | EU629214.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/10/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr M. McClelland(1). It was constructed by Dr C. Santiviago(2). (1) Department of Microbiology and Molecular Genetics, University of California, Irvine, USA (2) Department of Biochemistry and Molecular Biology, Faculty of Chemical and Farmaceutical Sciences, University of Chile, Santiago, Chile |
| Plasmid reference: | Santiviago et al., PLoS Pathog. 5 (2009), e1000477 [PMID: 19578432] [DOI: 10.1371/journal.ppat.1000477] |
| Related plasmid reference: | Porwollik et al., PLoS One. 9 (2014), e99820 [PMID: 25007190] [DOI: 10.1371/journal.pone.0099820] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli EC100D pir-116 |
| Host reference: | Metcalf et al., Gene 138 (1994), 1-7 [PMID: 8125283] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCLF4 (LMBP 9392) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. McClelland and was published in Santiviago et al., 2009. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.