Last data update: 24 January 2024 16:39 CET
Plasmid name: pCR3-Flag-hTRIF-RHIM (LMBP 5235)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human toll-like receptor (TLR) adaptor molecule 1 (TICAM1, hTRIF); fragment encoding amino acids 534-712 FLAG epitope tag; N-terminal |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Bovine growth hormone polyadenylation signal (BGH polyA) |
Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pCR3.V62-Met-Flag |
Further information: | The plasmid contains the Rip Homology Interaction Motif (RHIM) of hTRIF, which was cloned as an EcoRI-XhoI fragment into the EcoRI-XhoI opened pCR3.1-Flag vector. There is uncertainty about the presence of a second enhancer in the SV40 early promoter. The absence of a second enhancer in this promoter may cause bacterial leakage expression of the Tn5 neomycin resistance gene. This would cause transformed E. coli cells to be resistant to kanamycin, although growth should be reduced compared to growth on medium containing ampicillin. The nucleotide sequence of the hTRIF cDNA was obtained from Genbank (Accession number AB093555.1). Other name of the plasmid is pCR3-Flag-hTRIF RHIM. |
EMBL Accession number: | AB093555.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 04/09/2006 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr J. Tschopp(1). (1) Department of Biochemistry, University of Lausanne, Switzerland |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCR3-Flag-hTRIF-RHIM (LMBP 5235) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Tschopp. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.