Last data update: 24 January 2024 16:39 CET
Plasmid name: pCR3-hMLKL-T357E-S358D-EGFP (LMBP 10549)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human mixed lineage kinase domain like pseudokinase cDNA (MLKL, GeneID 197259); mutated sequence Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCR3-EGFPC-GW; pENTR3C-hMLKL-T357E-S358D-stop |
| Further information: | This Gateway expression vector was constructed by cloning the mutant human MLKL coding sequence, without a termination codon, into the pCR3-EGFPC-GW vector via Gateway LR recombination with pENTR3C-hMLKL-T357E-S358D-stop. The human MLKL coding sequence contains T357E and S358D mutations. The nucleotide sequence of the wild type human MLKL coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AK091708.1. The nucleotide sequence of the pCR3 vector backbone corresponds with EMBL Nucleotide Sequence Database accession number LT726803.1. Other name of the plasmid is pCR3-EGFPC-GW - hMLKL(FL) - T357E S358D - stop. |
| EMBL Accession number: | AK091708.1, view at EMBL, GenBank, DDBJ LT726803.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/11/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCR3-hMLKL-T357E-S358D-EGFP (LMBP 10549) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.