Last data update: 24 January 2024 16:39 CET
Plasmid name: pCR3-pro-IL-1B-D108A (LMBP 7534)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p7534.gb
(View with Genome Compiler) p7534.txt p7534.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interleukin 1 beta cDNA (Il1b, Il-1b, IL-1beta, GeneID 16176); mutated sequence Vesicular stomatitis virus glycoprotein (VSV-G) epitope |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCR3-pro-IL-1B |
| Further information: | The plasmid was created by mutating the mouse IL1B prosequence from pCR3-pro-IL-1B using overlap PCR, introducing a D108A mutation. The plasmid is meant to study the mouse IL1B prosequence cleavage site for cleavage by caspase-8. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726862.1. Referring to the depositor's sequence analysis of pGEM11mIL1B, codon 126 of mouse IL1B encodes for glycin (G) instead of arginin (R), and codon 266 consists of TCT instead of the wild type TCC. The nucleotide sequence of the wt mouse IL1B prosequence corresponds with the Genbank accession number NM_008361.3. Other name of the plasmid is pCR3-VSV-pro-IL1betaD108A. |
| EMBL Accession number: | NM_008361.3, view at GenBank LT726862.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/03/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI/KpnI, HincII, HindIII and NcoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by Dr J. Maelfait(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Maelfait et al., J. Exp. Med. 205 (2008), 1967-1973 [PMID: 18725521] [DOI: 10.1084/jem.20071632] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCR3-pro-IL-1B-D108A (LMBP 7534) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Maelfait et al., 2008. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.