Last data update: 24 January 2024 16:39 CET
Plasmid name: pCS2-KKR-Mir511-Tal2 (LMBP 9232)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
TALEN targeting mouse microRNA 511 (Mir511, Mirn511, mmu-mir-511) |
| Promoter: | Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer; sCMV-IE94 Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells Avian cells |
| Parental clone: | pCS2 |
| Further information: | The plasmid was constructed by cloning the TALEN against mouse Mir511 into the pCS2 vector. The plasmid uses a heterodimeric FokI endonuclease, containing E490K, I538K and H537R mutations (KKR). This plasmid is meant to be used with pCS2-ELD-Mir511-Tal1. Other name of the plasmid is pCS2+KKR + Mir511 Tal2#1. |
| EMBL Accession number: | - |
| Latest sequence update: | 01/03/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr T. Hochepied(1) (2) and Prof. Dr C. Libert(1) (2). It was constructed by J. D'Hont(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCS2-KKR-Mir511-Tal2 (LMBP 9232) is available at BCCM/GeneCorner. This plasmid was deposited by Dr T. Hochepied and Prof. Dr C. Libert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.