Last data update: 24 January 2024 16:39 CET
Plasmid name: pCS2-XTRAF4 (LMBP 6133)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6133.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Xenopus laevis TNF receptor associated factor 4 cDNA (TRAF4) |
| Promoter: | Simian cytomegalovirus immediate early promoter (CMV-IE94) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells Avian cells |
| Parental clone: | pCS2 |
| Further information: | The plasmid was constructed by cloning the Xenopus TRAF4 coding sequence, including 5' and 3' UTR regions, into the EcoRI opened pCS2 vector. The nucleotide sequence of the Xenopus TRAF4 cDNA corresponds with EMBL Nucleotide Sequence Database accession number BC080018.1. |
| EMBL Accession number: | BC080018.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/01/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Gerald H. Thomsen(1). (1) Department of Biochemistry and Cell Biology, Center for Developmental Genetics, Stony Brook University, Stony Brook, USA |
| Plasmid reference: | Kalkan et al., Mol. Biol. Cell 20 (2009), 3436-3450 [PMID: 19458200] [DOI: 10.1091/mbc.E08-03-0325] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCS2-XTRAF4 (LMBP 6133) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Gerald H. Thomsen and was published in Kalkan et al., 2009. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.