Last data update: 24 January 2024 16:39 CET
Plasmid name: pCUPZ/LacZ (LMBP 5794)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5794.gb
(View with Genome Compiler) p5794.txt p5794.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ) Histidine tag (His-tag); C-terminal Myc epitope; C-terminal |
| Promoter: | Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α) Synthetic prokaryotic EM7 promoter Saccharomyces cerevisiae copper-binding metallothionein promoter (CUP1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) Pichia pastoris alcohol oxidase 1 terminator (AOX1) |
| Selection marker: | Zeocin (zeo) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae |
| Parental clone: | pPICZ/LacZ |
| Further information: | The plasmid was created by removing the AOX1 promoter from pPICZ/LacZ via BglII-BstBI restriction. The CUP1 promoter was PCR amplified from S. cerevisiae gDNA and inserted as a BglII-BstBI fragment into the opened pPICZ/LacZ plasmid. |
| EMBL Accession number: | - |
| Latest sequence update: | 17/03/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/BglII, BglI/EcoRI, EcoRV and SacI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCUPZ/LacZ (LMBP 5794) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.