Last data update: 24 January 2024 16:39 CET
Plasmid name: pCXP34h-LmSP-12291 (LMBP 9836)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9836.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Leuconostoc mesenteroides sucrose phosphorylase cDNA (sp12291) Histidine tag (His-tag); N-terminal |
| Promoter: | Synthetic prokaryotic P34 promoter |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCXP34h |
| Further information: | The plasmid was constructed by cloning the L. mesenteroides sp12291 coding sequence into the pCXP34h vector. L. mesenteroides genomic DNA was obtained from ATCC (accession number ATCC 12291). The plasmid is meant for the constitutive expression of the sucrose phosphorylase from the lactic acid bacterium L. mesenteroides in E. coli BL21 DE3. The nucleotide sequence of the L. mesenteroides sp12291 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number D90314.1. Other name of the plasmid is pCXP34h-LmSP 12291. |
| EMBL Accession number: | D90314.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/04/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid is deposited by M. Diricks(1). It was constructed by Dr D. Aerts(1). (1) Centre of Expertise: Industrial Biotechnology and Biocatalysis, Faculty of Bioscience Engineering, University of Ghent, Ghent, Belgium |
| Plasmid reference: | Aerts et al., Eng. Life Sci. 11 (2011), 10-19 [DOI: 10.1002/elsc.201000065] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCXP34h-LmSP-12291 (LMBP 9836) is available at BCCM/GeneCorner. The plasmid is deposited by M. Diricks and was published in Aerts et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.