Last data update: 24 January 2024 16:39 CET
Plasmid name: pCXP34h-MsSP (LMBP 10226)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10226.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Meiothermus silvanus sucrose phosphorylase cDNA (SP) Histidine tag (His-tag); N-terminal |
| Promoter: | Synthetic prokaryotic P34 promoter |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCXP34h |
| Further information: | The plasmid was constructed by cloning the synthetic M. silvanus SP coding sequence into the pCXP34h vector. The plasmid is meant for the constitutive expression of the His-tagged M. silvanus SP in E. coli JW0987-4. The nucleotide sequence of the M. silvanus SP coding sequence was codon optimised for expression in E. coli. The nucleotide sequence of the wild type M. silvanus SP coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number CP002042.1. Other names of the plasmid are pCXP34h StSP and MsSP. |
| EMBL Accession number: | CP002042.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/12/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr T. Desmet(1). It was constructed by J. Franceus(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCXP34h-MsSP (LMBP 10226) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr T. Desmet. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.