Last data update: 24 January 2024 16:39 CET
Plasmid name: pCXP34h-ScLPMO10C-A143C-P183C-S73C-A115C (LMBP 10346)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10346.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Streptomyces coelicolor A3(2) lytic polysaccharide monooxygenase 10C cDNA (CelS2, LPMO10C, AA10C, SCO1188, SCG11A.19); mutated sequence Histidine tag (His-tag); C-terminal |
| Promoter: | Synthetic prokaryotic P34 promoter |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCXP34h-ScLPMO10C |
| Further information: | The plasmid was constructed by introducing S73C, A115C, A143C and P183C mutations into the S. coelicolor LPMO10C coding sequence of pCXP34h-ScLPMO10C via site-directed mutagenesis. The S. coelicolor LPMO10C coding sequence was codon-optimised for E. coli. The mutations introduced into the S. coelicolor LPMO10C coding sequence improve the stability of the encoded protein. The LPMO10C protein can be recovered from the periplasmic space. The nucleotide sequence of the wild type S. coelicolor LPMO10C coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AL939108.1. Other name of the plasmid is ScLPMO10C_A143C_P183C_S73C_A115C. |
| EMBL Accession number: | AL939108.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/02/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr T. Desmet(1). It was constructed by M. Tanghe(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | Tanghe et al., Protein Eng. Des. Sel. 3 (2017), 1-8 [PMID: 28338903] [DOI: 10.1093/protein/gzx014] |
| Related plasmid reference: | Aerts et al., Eng. Life Sci. 11 (2011), 10-19 [DOI: 10.1002/elsc.201000065] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCXP34h-ScLPMO10C-A143C-P183C-S73C-A115C (LMBP 10346) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr T. Desmet and was published in Tanghe et al., 2017. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.