Last data update: 24 January 2024 16:39 CET
Plasmid name: pCXP34h-SuSyAc-QMDRVRN (LMBP 10194)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10194.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Acidothiobacillus caldus sucrose synthase cDNA (SuSy); mutated sequence Histidine tag (His-tag); C-terminal |
| Promoter: | Synthetic prokaryotic P34 promoter |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCXP34h-SuSyAc |
| Further information: | The plasmid was constructed by introducing L636Q, L637M, K639R, T640V, V641R and A642N mutations into the A. caldus SuSy coding sequence of pCXP34h-SuSyAc via site-directed mutagenesis. The plasmid is meant for the constitutive expression of the His-tagged A. caldus mutant in E. coli BL21 DE3. This mutant has an increased affinity for UDP glucose. The nucleotide sequence of the A. caldus SuSy coding sequence was codon optimised for expression in E. coli. The nucleotide sequence of the wild type A. caldus SuSy cDNA corresponds with the EMBL Nucleotide Sequence Database accession number KP284426.1. Other names of the plasmid are SusyAc_QMDRVRN_pCXP34_Chis_plasmid and SuSyAc QMDRVRN. |
| EMBL Accession number: | KP284426.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/12/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr T. Desmet(1). It was constructed by M. Diricks(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | Diricks et al., Protein Eng. Des. Sel. 30 (2017), 141-148 [PMID: 27590052] [DOI: 10.1093/protein/gzw048] |
| Related plasmid reference: | Aerts et al., Eng. Life Sci. 11 (2011), 10-19 [DOI: 10.1002/elsc.201000065] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCXP34h-SuSyAc-QMDRVRN (LMBP 10194) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr T. Desmet and was published in Diricks et al., 2017. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.