Last data update: 24 January 2024 16:39 CET
Plasmid name: pDC304-MalP/H-HA (LMBP 5619)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP; MyD88 adapter-like, Mal); mutated isoform 2 Influenza HA epitope encoding the haemagglutinin tagging peptide; C-terminal |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pDC304-Mal-HA |
Further information: | The hMal coding sequence contains a Pro125His mutation. The nucleotide sequence of the wt hMal cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF406652.1. |
EMBL Accession number: | AF406652.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 04/07/2008 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr O'Neill(1). (1) Department of Biochemistry, Trinity College, Dublin, Ireland |
Plasmid reference: | Fitzgerald et al., Nature 413 (2001), 78-83 [PMID: 11544529] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDC304-MalP/H-HA (LMBP 5619) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr O'Neill and was published in Fitzgerald et al., 2001. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.