Last data update: 24 January 2024 16:39 CET
Plasmid name: pDEST-C-ECFP (LMBP 7877)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p7877.gb
(View with Genome Compiler) p7877.txt p7877.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (ECFP), C-terminal |
| Promoter: | Phaeodactylum tricornutum fucoxanthin chlorophyll a/c-binding protein B promoter (FcpB) Escherichia coli lac operon promoter Escherichia coli lac operon promoter; mutant (lacUV5) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Phaeodactylum tricornutum fucoxanthin chlorophyll a/c-binding protein A terminator (FcpA) |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Diatoms |
| Parental clone: | pDEST-OX; pECFP |
| Further information: | The plasmid was constructed by inserting the 839 bp PstI-SpeI fragment from pECFP, containing the ECFP coding sequence, into the PstI-SpeI opened pDEST-OX vector. pDEST-C-EYFP is a Gateway destination vector, containing the ccdB gene between the attenuator sites attR1 and attR2. It is meant for the creation of fusion proteins in diatoms, and contains a C-terminal ECFP reporter gene. The nucleotide sequence of the FcpB promoter corresponds with the EMBL Nucleotide Sequence Database accession number AF219942.1 except for an additional A nucleotide at position 920, a C instead of T in position 935, a missing GC nucleotide pair between positions 962 and 963 and an A instead of T in position 986. The nucleotide sequence of the FcpA terminator corresponds with the EMBL Nucleotide Sequence Database accession number AF219942.1. |
| EMBL Accession number: | AF219942.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/12/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HincII, KpnI, PstI/SpeI, PvuII and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr C. Bowler(1). (1) Institut de Biologie de l' Ecole Normale Supérieure (IBENS UMR8197 CNRS), Paris, France. |
| Plasmid reference: | Siaut et al., Gene 406 (2007), 23-35 [PMID: 17658702] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin. |
| Other culture collection numbers: | LMBP 07349 |
Refer in your Materials and Methods: |
pDEST-C-ECFP (LMBP 7877) is available at BCCM/GeneCorner. This plasmid was deposited by Dr C. Bowler and was published in Siaut et al., 2007. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.