Last data update: 24 January 2024 16:39 CET
Plasmid name: pDEST-EGFP-NLS-hRIPK3 (LMBP 10538)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p10538.gb
(View with Genome Compiler) p10538.txt p10538.pdf |
| Sequence analysis results Genecorner: |
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| Cloned DNA: |
Human receptor interacting serine/threonine kinase 3 cDNA (RIPK3, RIP3, GeneID 11035) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP),N-terminal SV40 large T-antigen nuclear localization signal (NLS); N-terminal |
| Promoter: | Escherichia coli β-lactamase promoter (amp) Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pDEST-EGFP; pENTR3C-NLS-hRIPK3 |
| Further information: | This Gateway expression vector was constructed by cloning the human RIPK3 coding sequence, preceded by the SV40 NLS sequence and including its termination codon, into the pDEST-EGFP vector via Gateway LR recombination with pENTR3C-NLS-hRIPK3. The nucleotide sequence of the human RIPK3 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF156884.1. Other name of the plasmid is pdest - EGFP - NLS - hRIP3(FL) + stop. |
| EMBL Accession number: | AF156884.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 21/02/2018 |
| Authenticity test: | The plasmid has been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDEST-EGFP-NLS-hRIPK3 (LMBP 10538) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.