Last data update: 24 January 2024 16:39 CET
Plasmid name: pDEST-LZRS-ires-eGFP (LMBP 11334)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11334.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) |
| Promoter: | Moloney mouse leukemia virus long terminal repeat (5' MMLV-LTR) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR) |
| Selection marker: | Ampicillin (amp) Puromycin (puro) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells |
| Further information: | This retroviral Gateway destination vector carries the EBNA1 gene which is a trans-acting factor that allows episomal maintenance of oriP-bearing plasmids. It is the only virally encoded protein necessary for plasmid maintenance. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/11/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Vandenabeele(1) (2). It was constructed by Dr L. Duprez(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 in E. coli; L2 in mammalian cells |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDEST-LZRS-ires-eGFP (LMBP 11334) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.