Last data update: 24 January 2024 16:39 CET
Plasmid name: pDEST15-mCtnna3 (LMBP 12570)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse catenin (cadherin associated protein), alpha 3 cDNA (Ctnna3, Catna3, Vr22, a-T-Ctn, GeneID 216033) Schistosoma japonicum Sj26 antigen glutathione S-transferase gene (GST) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation |
| Parental clone: | pDEST15 |
| Further information: | This Gateway Expression vector was constructed by cloning the mouse Ctnna3 coding sequence into the pDEST15 vector via Gateway LR recombination. Other name of the plasmid is pDEST15_maTctn. |
| EMBL Accession number: | - |
| Latest sequence update: | 14/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDEST15-mCtnna3 (LMBP 12570) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.