Last data update: 24 January 2024 16:39 CET
Plasmid name: pDMI,1 (LMBP 1594)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1594.gb
(View with Genome Compiler) p1594.txt p1594.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | pACYC184 |
| Further information: | Due to mutations in the lacI promoter (lacI(q)), the pDMI,1 repressor plasmid produces about 15 000 repressor molecules per cell in overnight cultures. The plasmid is compatible with all plasmids carrying the ColE1 origin of replication. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727579.1. The complete nucleotide sequence was kindly provided by Dr D. Stüber. |
| EMBL Accession number: | LT727579.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/02/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRV/NcoI, HindIII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by F. Hoffmann - La Roche LTD(1). (1) Basel, Switserland |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDMI,1 (LMBP 1594) is available at BCCM/GeneCorner. This plasmid was deposited by F. Hoffmann - La Roche LTD. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.