Last data update: 24 January 2024 16:39 CET
Plasmid name: pDest-LZRS-E-hA20-Ct-ZF1 (LMBP 5948)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p5948.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128); fragment Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) E-tag; N-terminal Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) |
Promoter: | Moloney mouse leukemia virus long terminal repeat (5' MMLV-LTR) |
Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR) |
Selection marker: | Puromycin (puro) |
Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
Host range: | Escherichia coli Mammalian cells |
Parental clone: | pDest-LZRS-IRES-GF; pEntry-2b-E-hA20-Ct-ZF1 |
Further information: | This retroviral Gateway expression vector was constructed by inserting the human A20 cDNA fragment from pEntry-2b-E-hA20-Ct-ZF1 into the vector pDest-LZRS-IRES-GFP via Gateway recombination with LR clonase. The nucleotide sequence of the hA20 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M59465.1. |
EMBL Accession number: | M59465.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 04/02/2009 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by Yanik Bruynooghe(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 in E. coli; L2 in mammalian cells |
Cultivation remark: | - |
Other culture collection numbers: | - |
Related website: | http://www.iresite.org/IRESite_web.php?page=view&entry_id=140 |
Refer in your Materials and Methods: |
pDest-LZRS-E-hA20-Ct-ZF1 (LMBP 5948) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.