Last data update: 24 January 2024 16:39 CET
Plasmid name: pEBG-p38 (LMBP 6227)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human mitogen-activated protein kinase 14 (MAPK14, p38) Schistosoma japonicum Sj26 antigen glutathione S-transferase gene (GST); N-terminal |
| Promoter: | Human elongation factor 1α promoter (EF1α) Escherichia coli lac operon promoter Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Human granulocyte macrophage colony stimulating factor polyadenylation signal (GM-CSF polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Bacteriophage M13 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pEBG |
| Further information: | Name mentioned in Carpentier et al. (1998) is pBEG-GSTp38. Other name of the plasmid is pEBG-GST-p38. |
| EMBL Accession number: | - |
| Latest sequence update: | 24/11/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr B. Mayer(1). (1) Genetics and Developmental Biology, UConn Health Center, Farmington, Connecticut, USA |
| Plasmid reference: | - |
| Related plasmid reference: | Sanchez et al., Nature 372 (1994), 794-798 [PMID: 7997269] Zanke et al., Curr. Biol. 6 (1996), 606-613 [PMID: 8805279] Carpentier et al., FEBS Lett. 425 (1998), 195-198 [PMID: 9559646] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEBG-p38 (LMBP 6227) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Mayer. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.