Last data update: 24 January 2024 16:39 CET
Plasmid name: pECFP-C-IkB (LMBP 8225)
New search | Print data sheet |
Price category: | Cat. 2 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p8225.gb |
Sequence analysis results Genecorner: |
Sanger: 127535.fasta Sanger: 127536.fasta |
Cloned DNA: |
Pig/human nuclear factor κB (NF-κB) inhibitor alpha hyrbid cDNA (IκBα, NFKBIA, MAD-3); human N-terminal fragment Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (ECFP), C-terminal |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli β-lactamase promoter (amp) Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pECFP-C1 |
Further information: | The plasmid was constructed by fusing the N-terminal 241 nt of human NFKBIA to the C-terminal 704 nt of pig NFKBIA, and cloning this hybrid coding sequence into the pECFP-C1 vector. The nucleotide sequence of the human NFKBIA cDNA fragment corresponds with the EMBL Nucleotide Sequence Database accession number BC004983.1. The nucleotide sequence of the pig NFKBIA cDNA fragment corresponds with the EMBL Nucleotide Sequence Database accession number Z21968.1. |
EMBL Accession number: | Z21968.1, view at EMBL, GenBank, DDBJ BC004983.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 13/03/2013 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr J.A. Schmid(1). (1) Center for Physiology and Pharmacology, Medical University Vienna, Vienna, Austria |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pECFP-C-IkB (LMBP 8225) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J.A. Schmid. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.