Last data update: 24 January 2024 16:39 CET
Plasmid name: pEF-SmIL1R1-His (LMBP 7861)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7861.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interleukin 1 receptor 1 cDNA (IL1R1); soluble form Myc epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human elongation factor 1α promoter (EF1α) Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Synthetic prokaryotic EM7 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Blasticidin (bsd) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pEF6/Myc-HisA |
| Further information: | The plasmid was created by PCR-amplifying the soluble (extracellular) part of the mouse IL1R1 from L929 mouse cell line cDNA and cloning it as an EcoRI/XbaI fragment into the pEF6/Myc-HisA vector. The plasmid is meant for expression of soluble mouse IL1R1 in mammalian cells. The expressed protein is secreted into the medium and can be detected or purified via the C-terminal myc/His tag. The nucleotide sequence of the mouse soluble IL1R1 cDNA corresponds with the Genbank accession number NM_008362.2. |
| EMBL Accession number: | NM_008362.2, view at GenBank |
| Latest sequence update: | 07/09/2012 |
| Sequence detail: | Primers used to amplify the soluble (extracellular) part of the mouse IL-1R1:
Forward: 5' TTCGAATTC.ATG.GAG.AAT.ATG.AAA.GTG.CTA.CTG
EcoRI ***
Reverse: 5' ATTTCTAGA.CTT.GAA.GTC.AGG.GAC.TGG.GTA
XbaI
***: start codon.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr H. Braun(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEF-SmIL1R1-His (LMBP 7861) is available at BCCM/GeneCorner. This plasmid was deposited by Dr H. Braun and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.