Last data update: 24 January 2024 16:39 CET
Plasmid name: pEGFPC2-haEctn(69-3454) (LMBP 4552)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4552.gb
(View with Genome Compiler) p4552.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Human αE-catenin cDNA (aEctn, CTNNA1) |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli β-lactamase promoter (amp) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pDR2αECTN; pEGFP-C2 |
| Further information: | The plasmid was constructed as follows: cDNA of the human αE-catenin (haEctn) was isolated from a human fetal kidney cDNA library by the use of an αE-catenin-specific probe. A fragment of 3690 nucleotides, containing the complete coding sequence, the 3 UTR region and the 3' flanking region (exon 18) was inserted between the EcoRI and SalI sites of pEGFP-C2. As a result haEctn was N-terminally fused to the enhanced red-shifted variant of the Aequorea victoria green fluorescent protein gene (EGFP). The fusion gene is expressed under control of the human cytomegalovirus immediate early promoter (hCMV-IE) and enhancer. The haEctn sequence corresponds with the EMBL Nucleotide Sequence Database accession numbers D14705 (version SV 1) and AF102803 (version SV 1). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727105.1. Other name of the plasmid is pEGFP2-hαTctn(69-3454). |
| EMBL Accession number: | AF102803, view at EMBL, GenBank, DDBJ LT727105.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/07/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/SalI, EcoRI and NcoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr B. Janssens (1) (2) and Prof. Dr F. Van Roy (1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Janssens et al., J. Cell Sci. 114 (2001), 3177-3188 [PMID: 11590244] |
| Related plasmid reference: | Lippincott-Schwartz et al., Science 300 (2003), 87-91 [PMID: 12677058] Furukawa et al., Cytogenet. Cell Genet. 65 (1994), 74-78 [PMID: 8404069] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (30 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEGFPC2-haEctn(69-3454) (LMBP 4552) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. Janssens and Prof. Dr F. Van Roy and was published in Janssens et al., 2001. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.