Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR-mRIP1-ID-V5His (LMBP 5311)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5311.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse receptor TNFRSF-interacting serine-threonine kinase 1 cDNA (Ripk1, Rip1, Rinp, GeneID 19766); intermediate domain (Ripk1-ID) V5 epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR3C; pFBneo-mRIP1-ID-V5His |
| Further information: | This Gateway entry vector was constructed by inserting a 846 bp BamHI-XhoI fragment, containing the mouse RIP1-ID-V5His coding sequence, into the BamHI-XhoI cleaved pENTR3C vector. The mouse RIP1-ID-V5His was obtained by removing this insert from pFBneo-mRIP1-ID-V5His by BamHI/XhoI digest. The nucleotide sequence of the mRIP1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC058162.1. |
| EMBL Accession number: | BC058162.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 19/08/2010 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI, BamHI/XhoI, EcoRI/Nhe andI EcoRV. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by N. Festjens(1) (2) and Prof. Dr P. Vandenabeele(1) (2). It was constructed by N. Festjens and I. Vanoverberghe(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR-mRIP1-ID-V5His (LMBP 5311) is available at BCCM/GeneCorner. This plasmid was deposited by N. Festjens and Prof. Dr P. Vandenabeele . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.