Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR201-slrP (LMBP 6728)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6728.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Salmonella enterica serovar Typhimurium LT2 leucine-rich repeat protein cDNA (slrP) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pDONR201 |
| Further information: | This Gateway entry vector was constructed cloning the PCR amplified slrP cDNA into the pDONR201 vector via Gateway recombination. The nucleotide sequence of the slrP cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AE006468.1. |
| EMBL Accession number: | AE006468.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/08/2010 |
| Sequence detail: | Primers used to isolate the SlrP cDNA:
Forward primer: 5' GGGGACAAGTTTGTACAAAAAAGCAGGCTTG.TTT.AAT.ATT.ACT.AAT.ATA.CAA.TCT.ACG.GCA
AA2
Reverse primer: 5' GGGGACCACTTTGTACAAGAAAGCTGGGTG.CTA.TCG.CCA.GTA.GGC.GCT.CAT
+++
+++: termination codon
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI/PstI, HpaI, PvuII and SspI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by W. De Schamphelaire(1) and Prof. Dr R. Beyaert(1). (1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR201-slrP (LMBP 6728) is available at BCCM/GeneCorner. This plasmid was deposited by W. De Schamphelaire and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.