Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR207-hTMBIM6-dC (LMBP 10248)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10248.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human transmembrane BAX inhibitor motif containing 6 cDNA (TMBIM6, TEGT, BAXI1, BI-1, GeneID 7009); fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Gentamicin (Gm) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pDONR207 |
| Further information: | This Gateway Entry plasmid was constructed by PCR amplifying the human TMBIM6 coding sequence, without the 10 C-terminal codons and the termination codon, and cloning it into the pDONR207 vector via Gateway BP recombination Other names of the plasmid are pENTR207-BaxInhibitor(delta C-term part) and pDONR207-BaxInhibitor-DeltaC. |
| EMBL Accession number: | - |
| Latest sequence update: | 09/08/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr S. Grootjans(1)(2) and Prof. Dr P. Vandenabeele(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + gentamicin (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR207-hTMBIM6-dC (LMBP 10248) is available at BCCM/GeneCorner. The plasmid was deposited by Dr S. Grootjans and Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.