Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR207-hVCL-IN2+VH3+Tail (LMBP 12563)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human vinculin cDNA (VCL, GeneID 7414); fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Gentamicin (Gm) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pDONR207 |
| Further information: | This Gateway entry vector was constructed by cloning a fragment of the human VCL coding sequence into the pDONR207 vector via Gateway BP recombination. The plasmid encodes the linker peptide IN2, the head domain VH3 and the tail domain of human VCL. Other name of the plasmid is pDONR207 hVinculin IN2+VH3+Tail cl5. |
| EMBL Accession number: | - |
| Latest sequence update: | 06/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + gentamicin (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR207-hVCL-IN2+VH3+Tail (LMBP 12563) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.