Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR221-hHOIL1-R165A (LMBP 9155)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human RANBP2-type and C3HC4-type zinc finger containing 1 cDNA (RBCK1, HOIL1, RBCK2, RNF54, UBCE7IP3, XAP4, ZRANB4, GeneID 10616); mutated sequence |
Promoter: | Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T2 terminator Escherichia coli rrnB operon T1 terminator |
Selection marker: | Neomycin (neo; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pDONR221; pCDNA3zeo-HOIL-R165A |
Further information: | The plasmid was contructed by PCR amplifying the human RBCK1 coding sequence from pCDNA3zeo-HOIL-R165A and cloning it into the pDONR221 vector via Gateway BP recombination. The MALT1 cleavage site of the human RBCK1 was mutated by an R165A mutation. The nucleotide sequence of the wild type human RBCK1 coding sequence corresponds with Genbank accession number NG_033233.1. Other name of the plasmid is pENTR221-HOIL-1L-R165A. |
EMBL Accession number: | AF420371.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 20/04/2020 |
Sequence detail: | Primers used to amplify the human RBCK1 coding sequence: Forward: attB1-cc.ATG.GAC.GAG.AAG.ACC.AAG.AAA.G Reverse: attB2-GTGGCAGTTCTGACAGCTTG |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR221-hHOIL1-R165A (LMBP 9155) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.