Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR221-hOPTN (LMBP 9148)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | - |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human optineurin (OPTN, GLC1E, FIP2, HYPL, FIP-2, TFIIIA-INTP, NRP, HIP7, GeneID 10133) |
Promoter: | Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T2 terminator Escherichia coli rrnB operon T1 terminator |
Selection marker: | Neomycin (neo; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pDONR221; pCAGGS-E-hOPTN |
Further information: | The plasmid was contructed by PCR amplifying the human OPTN coding sequence from pCAGGS-E-hOPTN and cloning it into the pDONR221 vector via Gateway BP recombination. The nucleotide sequence of the human OPTN coding sequence corresponds with EMBL Nucleotide Sequence Database accession number AF420371.1. Other name of the plasmid is pENTR221-hOPTN-WT. |
EMBL Accession number: | AF420371.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 16/04/2020 |
Sequence detail: | Primers used to amplify the human OPTN coding sequence: Forward: attB1-ccATGTCCCATCAACCTCTCAGCT Reverse: attB2-TTAAATGATGCAATCCATCACG |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR221-hOPTN (LMBP 9148) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.