Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR3C-GFP-LC3 (LMBP 7287)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7287.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Rattus norvegicus microtubule-associated protein 1 light chain 3 beta cDNA (LC3) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), N-terminal |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR3C |
| Further information: | This Gateway entry vector was created by isolating the ccdB-gene from pENTR3C as a KpnI-EcoRI fragment and replacing it with the EGFP-LC3 cassette. The nucleotide sequence of the LC3 cds corresponds with the EMBL Nucleotide Sequence Database accession number BC083556 .1. |
| EMBL Accession number: | BC083556.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/06/2011 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BanI, BanII, EcoRI/KpnI and PvuI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by E. Wirawan(1) (2) and Prof. Dr P. Vandenabeele(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR3C-GFP-LC3 (LMBP 7287) is available at BCCM/GeneCorner. This plasmid was deposited by E. Wirawan and Prof. Dr P. Vandenabeele . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.