Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR3C-strep-hMLKL(211-471)-L291P (LMBP 8707)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8707.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human mixed lineage kinase domain like pseudokinase cDNA (MLKL, GeneID 197259); mutated fragment Strep-tag II; N-terminal |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR3C |
| Further information: | This Gateway entry plasmid was constructed by introducing an L291P mutation in the human MLKL coding sequence, and cloning a C-terminal fragment, consisting of codons 211 up to and including 471, into the pENTR3C vector. The human MLKL fragment in this plasmid does not include a termination codon, allowing for C-terminal tagging. An N-terminal Strep-tag II was fused to the human MLKL coding sequence via PCR. The nucleotide sequence of the wild type human MLKL coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AK091708.1. Other name of the plasmid is pENTR3C-strep-hMLKL(211-471)-L291P-stop. |
| EMBL Accession number: | AK091708.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/10/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr Y. Dondelinger(1) (2) and Prof. Dr M. Bertrand(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Dondelinger et al., Cell Rep. 7 (2014), 971-981 [PMID: 24813885] [DOI: 10.1016/j.celrep.2014.04.026] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR3C-strep-hMLKL(211-471)-L291P (LMBP 8707) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Y. Dondelinger and Prof. Dr M. Bertrand . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.