Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR3C_SARS-CoV-2_ORF9c-noStop (LMBP 12499)
| New search | Print data sheet |
| Price category: | Cat. 2 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p12499.gb |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) open reading frame 9c cDNA (ORF9c) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T2 terminator Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR3C |
| Further information: | This Gateway Entry plasmid was constructed by cloning the SARS-CoV-2 ORF9c coding sequence, without a termination codon, into the pENTR3C vector. |
| EMBL Accession number: | NC_045512.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 23/06/2021 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data were unfortunately incomplete. The NGS sequence data also still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by S. Verdonck and Dr J. Maelfait (1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR3C_SARS-CoV-2_ORF9c-noStop (LMBP 12499) is available at BCCM/GeneCorner. This plasmid was deposited by S. Verdonck and Dr J. Maelfait . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.