Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTRYmRIP4+stop (LMBP 5345)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5345.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse receptor-interacting serine-threonine kinase 4 cDNA (Ripk4, ANKK2, Ankrd3, DIk, PKK, RIP4) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR3C |
| Further information: | This Gateway entry vector is a high copy number Gateway ENTRY vector containing mouse RIP4 with a termination codon. The nucleotide sequence of the mRIP4 cDNA corresponds with EMBL Nucleotide Sequence Database accession number BC057871.1. |
| EMBL Accession number: | BC057871.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/10/2006 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Philippe De Groote (2012) |
| Related plasmid reference: | Bertrand et al., PLoS One. 6 (2011), e22356 [PMID: 21931591] [DOI: 10.1371/journal.pone.0022356] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTRYmRIP4+stop (LMBP 5345) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. De Groote and Prof. Dr W. Declercq and was published in Philippe De Groote, 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.