Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-15b_HIS_hCSF-1 (LMBP 9807)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human colony stimulating factor 1 cDNA (CSF1, M-CSF, MCSF); fragment Histidine tag (His-tag); N-terminal Escherichia coli lac repressor gene (lacI) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-15b |
| Further information: | The plasmid was constructed by cloning an N-terminal fragment of the human CSF1 coding sequence, containing codons 1 up to and including 149, into the NdeI/BamHI opened pET-15b vector. The nucleotide sequence of the human CSF1 coding sequence corresponds with the Genbank accession number NM_172212.2. |
| EMBL Accession number: | NM_172212.2, view at GenBank |
| Latest sequence update: | 23/06/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr S. Savvides (1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) Inflammation Research Center, VIB, Ghent, Belgium |
| Plasmid reference: | Felix et al., Structure 23 (2015), 1621-1631 [PMID: 26235028] [DOI: 10.1016/j.str.2015.06.019] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-15b_HIS_hCSF-1 (LMBP 9807) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr S. Savvides and was published in Felix et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.