Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-21a-ArUGAE (LMBP 12135)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
NGS: b02-gc-may2021.fasta |
| Cloned DNA: |
Agrobacterium radiobacter UDP-glucuronate 5-epimerase cDNA (UGAE, UGA5E) Histidine tag (His-tag); C-terminal Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-21a |
| Further information: | The plasmid was constructed by cloning the synthetic A. radiobacter UGAE coding sequence, codon optimized for E. coli, into the pET-21a vector. The plasmid is meant for the constitutive expression of A. radiobacter UGAE in E. coli BL21. The nucleotide sequence of the wild-type A. radiobacter UGAE cDNA corresponds with the EMBL Nucleotide Sequence Database accession number CP000628.1. Other name of the plasmid is ArUGAE. |
| EMBL Accession number: | CP000628.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/08/2020 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by O. Gevaert(1) and Prof. Dr T. Desmet(1). It was purchased from GeneArt(2). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium (2) www.thermofisher.com/geneart |
| Plasmid reference: | Gevaert et al., Catalysts 10 (2020), 222 [DOI: 10.3390/catal10020222] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-21a-ArUGAE (LMBP 12135) is available at BCCM/GeneCorner. The plasmid was deposited by O. Gevaert and Prof. Dr T. Desmet and was published in Gevaert et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.