Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-21a-BaSP-YGQF (LMBP 12110)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Bifidobacterium adolescentis sucrose phosphorylase cDNA (SP, GeneID 56674126); mutated sequence Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) Histidine tag (His-tag); C-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-21a |
| Further information: | The plasmid was constructed by cloning the synthetic mutated B. adolescentis SP coding sequence, codon optimized for E. coli, into the pET-21a vector. The plasmid encodes for a mutant of B. adolescentis SP, containing R135Y, D342G, Y344Q, Q345F, Q331E, R393N, D445P, D446T, Q460E and E485H mutations. The plasmid is meant for the constitutive expression of thermostable sucrose phosphorylase in E. coli BL21 for the production of nigerose. Other name of the plasmid is BaSP YGQF (nigerose). |
| EMBL Accession number: | - |
| Latest sequence update: | 03/06/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by J. Franceus(1) and Prof. Dr T. Desmet(1). It was purchased from GeneArt(2). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium (2) www.thermofisher.com/geneart |
| Plasmid reference: | Franceus et al., Chem. Commun. (Camb.) 55 (2019), 4531-4533 [PMID: 30924472] [DOI: 10.1039/C9CC01587F] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-21a-BaSP-YGQF (LMBP 12110) is available at BCCM/GeneCorner. The plasmid was deposited by J. Franceus and Prof. Dr T. Desmet and was published in Franceus et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.