Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-21a-CrUGT-SA (LMBP 10214)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10214.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Capsella rubella salicylic acid β-glucosyltransferase cDNA (UGT-SA) Escherichia coli lac repressor gene (lacI) Histidine tag (His-tag); C-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-21a |
| Further information: | The plasmid was constructed by cloning the synthetic C. rubella UGT-SA coding sequence into the pET-21a vector. The plasmid is meant for the constitutive expression of the His-tagged C. rubella UGT-SA in E. coli BL21 DE3. The nucleotide sequence of the C. rubella UGT-SA coding sequence was codon optimised for expression in E. coli. The nucleotide sequence of the wild type C. rubella UGT-SA coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number KB870808.1. Other names of the plasmid are pET21a_UGT-SA from Capsella rubella and Cr UGT-SA. |
| EMBL Accession number: | KB870808.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/12/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr T. Desmet(1). It was constructed by G. Dewitte(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | Dewitte et al., J. Biotechnol. 233 (2016), 49-55 [PMID: 27378621] [DOI: 10.1016/j.biotec.2016.06.034] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-21a-CrUGT-SA (LMBP 10214) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr T. Desmet and was published in Dewitte et al., 2016. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.