Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-21a-CsCE (LMBP 12123)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Caldicellulosiruptor saccharolyticus cellobiose 2-epimerase cDNA (ce) Histidine tag (His-tag); C-terminal coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-21a |
| Further information: | The plasmid was constructed by cloning the synthetic C. saccharolyticus ce coding sequence, codon optimized for E. coli, into the pET-21a vector. The plasmid is meant for the constitutive expression of C. saccharolyticus CE (UniProtID A4XGA6) in E. coli BL21. The C. saccharolyticus CE enzyme can be used for the conversion of galactose to talose. Other name of the plasmid is pET21a_CsCE_WT. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/08/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by S. Van Overtveldt(1) and Prof. Dr T. Desmet(1). It was purchased from GeneArt(2). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium (2) www.thermofisher.com/geneart |
| Plasmid reference: | Van Overtveldt et al., Molecules 23 (2018), 2519 [PMID: 30275414] [DOI: 10.3390/molecules23102519] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-21a-CsCE (LMBP 12123) is available at BCCM/GeneCorner. The plasmid was deposited by S. Van Overtveldt and Prof. Dr T. Desmet and was published in Van Overtveldt et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.