Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-21a-HaNALase (LMBP 10217)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10217.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Helicobacter acinonychis N-acetyllactosamine synthase cDNA (NALase) Escherichia coli lac repressor gene (lacI) Histidine tag (His-tag); C-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-21a |
| Further information: | The plasmid was constructed by cloning the synthetic H. acinonychis NALase coding sequence into the pET-21a vector. The plasmid is meant for the constitutive expression of the His-tagged H. acinonychis NALase in E. coli BL21 DE3. The nucleotide sequence of the H. acinonychis NALase coding sequence was codon optimised for expression in E. coli. The nucleotide sequence of the wild type H. acinonychis NALase coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AM260522.1. Other names of the plasmid are pET21a_NALase from Helicobacter acinonichyis and Ha NALase. |
| EMBL Accession number: | AM260522, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/12/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr T. Desmet(1). It was constructed by G. Dewitte(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-21a-HaNALase (LMBP 10217) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr T. Desmet. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.