Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-21a-TtGalE-I189A-V200E-L202W (LMBP 12133)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Thermus thermophilus NAD-dependent epimerase/dehydratase family protein cDNA (TTH_RS03070, GalE, GeneID 3168492); mutated sequence Histidine tag (His-tag); C-terminal Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET-21a |
| Further information: | The plasmid was constructed by cloning the synthetic mutated T. thermophilus GalE coding sequence into the pET-21a vector. The plasmid is meant for the constitutive expression of mutant T. thermophilus GalE in E. coli BL21. The T. thermophilus GalE coding sequence contains I189A, V200E and L202W mutations. Other name of the plasmid is pET21a_TtGalE_I189A_V200E_L202W. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/08/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by S. Van Overtveldt(1) and Prof. Dr T. Desmet(1). It was purchased from GeneArt(2). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium (2) www.thermofisher.com/geneart |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-21a-TtGalE-I189A-V200E-L202W (LMBP 12133) is available at BCCM/GeneCorner. The plasmid was deposited by S. Van Overtveldt and Prof. Dr T. Desmet. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.