Last data update: 24 January 2024 16:39 CET
Plasmid name: pET-29a-S-hUBE2N-His (LMBP 9069)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9069.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human ubiquitin conjugating enzyme E2 N cDNA (UBE2N, UBC13, UbcH-ben) Escherichia coli lac repressor gene (lacI) S-tag; N-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pFLAG-CMV2-UbcH13; pET-29a(+) |
| Further information: | The plasmid was constructed by cloning the human UBE2N coding sequence from pFLAG-CMV2-UbcH13 into the NdeI/XhoI opened pET-29a(+) vector. This plasmid contains the T7 gene 10 promoter whose transcription is initiated by T7 RNA polymerase, making the vector useful for in vitro preparation of single-stranded RNAs from the cloned human UBE2N gene. Other name of the plasmid is UBCH13-6xHis. |
| EMBL Accession number: | - |
| Latest sequence update: | 05/04/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr R. Beyaert(1)(2). It was constructed by Dr. B. Van Raam(3) (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium (3) BioMed X Innovation Center, Heidelberg, Germany |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET-29a-S-hUBE2N-His (LMBP 9069) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.