Last data update: 24 January 2024 16:39 CET
Plasmid name: pET15b-His-mIL9(19-144) (LMBP 12811)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interleukin 9 cDNA (Il9, Il-9, P40, GeneID 16198); fragment Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) Histidine tag (His-tag); N-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET15b |
| Further information: | The plasmid was constructed by cloning a fragment of the mouse Il9 coding sequence, consisting of codons 19 up to and including 144, into the NdeI/BamHI opened pET15b vector. The plasmid is intended for expression of the mouse IL9 fragment in E. coli. The mouse IL9 is expressed with an N-terminal HIS-tag, which can be removed via a thrombin cleavage site. Other name of the plasmid is pET15b_HIS_mIL-9. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/11/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr S. Savvides(1)(2). It was constructed by Dr T. De Vos(1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET15b-His-mIL9(19-144) (LMBP 12811) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr S. Savvides. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.